Adding to what Fredledingue is saying here but basically it depends on the stock you are trading.
A stock like Apple (AAPL), 1000 shares will not affect anything, but if you are trading a small cap stock that say only moves volume (total shares traded) of 100,000 per day well then your 1000 shares bought at the market at the same time may move the stock price a bit.
A great way to see if your purchase will move the stock is to pull up a level II quote and see exactly how many shares are available at the current bid or ask

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